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Poster apresentado nas IV Jornadas de Genética, realizadas na UTAD, Vila Real, nos dias 1,2 e 3 de Março de 2012.
Disseminated Mycobacterium avium subsp. paratuberculosis (MAP) infections were found in two Eurasian otters (Lutra lutra, L. 1758) killed by vehicular trauma in February and March 2010 in Castelo Branco, Portugal. At postmortem examination, the organs showed no significant gross alterations; however, microscopically, both animals had diffuse lymphadenitis with macrophage infiltration and deposition of hyaline material in the center of the lymphoid follicles. Acid-fast organisms were isolated from gastrointestinal tissue samples via bacteriologic culture. These organisms were identified as M. avium subsp. paratuberculosis by IS900 polymerase chain reaction (PCR). Additionally, direct IS900 PCR-positive results were obtained for multiple organs of both animals. This is the first report of MAP infection of otters in Portugal.
Deteção molecular de Mycobacterium avium subsp. paratuberculosis em duas lontras (Lutra lutra, Linnaeus, 1758).
Paratuberculosis or Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. The present work is part of a wider set of studies designed to assess the prevalence of paratuberculosis in free ranging red deer (Cervus elaphus). With that purpose, 877 free-ranging red deer legally hunted in the Centre-eastern Portugal were submitted to necropsy and sampled for molecular methods, microbiology and histopathology. Thirty-seven (4.2%) kidneys revealed acid-fast bacilli when screened with the Ziehl-Neelsen technique. Map was detected by IS900 polymerase chain reaction (PCR) in thirty (81.1%) of the Ziehl-Neelsen positive kidneys. Subsequent PCR and/or culture from the different organs of the 37 examined animals allowed us to detect 86.4% (32 animals) infected red deer. Our results suggest that renal involvement in Map infected deer may be underdiagnosed and thus the routine examination of this organ and its inclusion in PCR techniques designed for Map detection could substantially improve the diagnostic of paratuberculosis in red deer.
O diagnóstico histoquímico de micobacterioses, através da visualização de bacilos álcool-ácido resistentes (BAAR), em esfregaços corados mediante a técnica de Ziehl-Neelsen, a partir de amostras de tecidos, é considerada como uma boa técnica de diagnóstico presuntivo. Neste estudo pretendeu-se avaliar a frequência de visualização de BAAR em rim e gânglios linfáticos mesentéricos com lesões granulomatosas em veados (Cervus elaphus) e javalis (Sus scrofa). Os esfregaços de cada órgão foram efectuados sobre uma lâmina de vidro e as preparações foram coradas de acordo com o método de Ziehl-Neelsen.
Dos 91 esfregaços de gânglios mesentéricos analisados, 46 (50,5%) mostraram BAAR compatíveis, sendo classificados como positivos. Vinte e dois esfregaços de rim dos 25 analisados (88%) apresentaram BAAR. Dos 25 animais, em que foram observados simultaneamente esfregaços de gânglios mesentéricos e rim, 15 (60%) apresentaram resultados coincidentes. Em javalis, a frequência de esfregaços, classificados como positivos, foi de 76,2% (32/42) e em veados de 48,6% (36/74).
Neste estudo, o rim revelou-se o órgão com maior frequência de visualização de BAAR, e o javali como a espécie animal que apresentou maior percentagem de resultados positivos.
ranulomatous sesions and Mycobacterium avium subsp. paratuberculosis in Portuguese wild boars (Sus scrofa).
Diffuse lymphadenitis and disseminated Mycobacterium avium subsp. paratuberculosis infection in two wild Eurasian otters (Lutra lutra L. 1758).
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Diffuse lymphadenitis and disseminated Mycobacterium avium subsp. paratuberculosis infection in two wild eurasian otters (Lutra lutra L. 1758).
Renal lesions in deer (Cervus elaphus) - Mycobacterium avium subsp. paratuberculosis involvelment.
Granulomatous lesions and Mycobacterium avium subsp. paratuberculosis in portuguese wild boars (Sus scrofa).
