In vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovora

article

Mendes, Rafael
Regalado, Laura
Luz, J.P.
Tassi, Natália
Teixeira, Cátia
Gomes, P.
Tavares, Fernando
Santos, Conceição

MDPI

MENDES, R.J [et al.] (2021) - In vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovora. Biomolecules. 11:4, p. 554-571. DOI https://doi.org/10.3390/biom11040554

10.3390/biom11040554

In vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovora

Antimicrobial activity
CA-M
Dhvar-5
D4E1
Fire blight
Flow cytometry
Membrane permeabilization
RW-BP100

info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F133519%2F2017/PT
FEDER UIDB/50006/2020
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBAA-AGR%2F31798%2F2017/PT
COMPETE 2020

2021-04-23T11:31:25Z
2021-04-23T11:31:25Z
2021

Fire blight is a major pome fruit trees disease that is caused by the quarantine phytopathogenic Erwinia amylovora, leading to major losses, namely, in pear and apple productions. Nevertheless, no effective sustainable control treatments and measures have yet been disclosed. In that regard, antimicrobial peptides (AMPs) have been proposed as an alternative biomolecule against pathogens but some of those AMPs have yet to be tested against E. amylovora. In this study, the potential of five AMPs (RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) together with BP100, were assessed to control E. amylovora. Antibiograms, minimal inhibitory, and bactericidal concentrations (minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), growth and IC50 were determined and membrane permeabilization capacity was evaluated by flow cytometry analysis and colony-forming units (CFUs) plate counting. For the tested AMPs, the higher inhibitory and bactericidal capacity was observed for RW-BP100 and CA-M (5 and 5–8 M, respectively for both MIC and MBC), whilst for IC50 RW-BP100 presented higher efficiency (2.8 to 3.5 M). Growth curves for the first concentrations bellow MIC showed that these AMPs delayed E. amylovora growth. Flow cytometry disclosed faster membrane permeabilization for CA-M. These results highlight the potential of RW-BP100 and CA-M AMPs as sustainable control measures against E. amylovora.
info:eu-repo/semantics/publishedVersion

openAccess
http://creativecommons.org/licenses/by/4.0/

eng