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The valorisation of agro-industrial by-products can be used as an alternative source for producing healthy food ingredients and materials. The volatile profile measured by headspace solid-phase microextraction-gas chromatography-mass spectrometry, mechanical, antioxidant and antibacterial properties of biodegradable cassava starch film extruded with the addition of different percentages of apple pomace (AP) by-product were evaluated. Characteristic volatile compounds were identified in the samples, such as α-farnesene, sulcatone and ethylphenyl propanoate. The film containing 8% AP had higher total phenolic content (3.32 mg GAE/g, Gallic acid equivalent) and antioxidant activity (2.78 mmol Trolox/g) than the control film (0.71 mg GAE/g and 1.03 mmol Trolox/g). The minimum inhibitory concentration against Staphylococcus aureus, Salmonella thyphimurium, and Escherichia coli for the films containing AP was higher than 12.5 mg/mL for all species. The results suggest that the antioxidant and antibacterial activities and mechanical properties, are dependent on the quantity of the AP added to films. Due to its antioxidant activity and mechanical properties, the AP could have real potential use as a functional ingredient in biodegradable packaging with the advantage of having good antibacterial properties.

The development of new materials environmentally friendly has become an important market niche for the food industry. The agro-industrial wastes and by-products can be an alternative for the production of biodegradable food packaging. The work aimed to produce biodegradable plastic film extruded with antioxidant and anti- bacterial properties by the joining of cassava starch and Brazilian propolis by-product (BPB). The volatile profile of BPB, Brazilian propolis by-product film (BPBF), and control film (CF) were analyzed by gas chromatography. The mechanical, antioxidant, and antibacterial properties of the films were also assessed. Eighty-seven volatile compounds were detected with aldehydes and terpenoids predominating in the samples. The major terpenoid detected in the samples was the 2-pinen-10-ol followed by α-copaene. Benzaldehyde and benzenepropanoic acid ethyl ester were the major aldehyde and ester classes present in the films. The BPBF exhibited promising me- chanical properties showing the highest Elasticity modulus (11.96 Mpa) and excellent antioxidant (8.45 mmol Trolox equivalent/g) and antibacterial (12.5 mg/mL against Staphylococcus aureus and Salmonella Typhimurium) activities. The valorization of agro-industrial by-products following the current trends to environmental care can be a sustainable alternative for the development of a plastic into which the propolis by-product is incorporated in biodegradable packaging.