Antioxidant, anti-inflammatory, and analgesic activities of agrimonia eupatoria L. Infusion

article

Santos, Telmo N.
Costa, Gustavo
Ferreira, J. Pinto
Liberal, Joana
Francisco, Vera
Paranhos, António
Cruz, Maria T.
Castelo-Branco, M.
Figueiredo, I. Vitória
Batista, M. Teresa

Hindawi Publishing Corporation

SANTOS, Telmo N. [et al.] - Antioxidant, anti-inflammatory, and analgesic activities of agrimonia eupatoria L. Infusion. Evidence-Based Complementary and Alternative Medicine. ISSN. 1741-427X. (2017) p. 1-13

1741-427X

10.1155/2017/8309894

Antioxidant, anti-inflammatory, and analgesic activities of agrimonia eupatoria L. Infusion

Agrimonia eupatoria L.
Antioxidant activity
Anti-inflammatory activity
Analgesic activity

FEDER/COMPETE (FCOMP-01-0124-FEDER-011096)
FCT Project PTDC/SAU-FCF/105429/2008
FCT Project PEst-OE/SAU/UI0177/2011

2017-11-06T15:04:44Z
2017-11-06T15:04:44Z
2017-04-12

Agrimony (Agrimonia eupatoria L.) (Ae) is used in traditional medicine to treat inflammatory and oxidative related diseases. Therefore, this study focuses on the anti-inflammatory and analgesic potential of Ae infusion (AeI). Phenolic compounds characterization was achieved by HPLC-PDA-ESI/MS (n) . To evaluate antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, and SNAP assays were used. In vitro anti-inflammatory activity of AeI was investigated in LPS-stimulated macrophages by measuring the NO production. In vivo anti-inflammatory activity was validated using the mouse carrageenan-induced paw edema model. Peripheral and central analgesic potential was evaluated using the acetic acid-induced writhing and hot-plate tests, respectively, as well as the formalin assay to assess both activities. The safety profile was disclosed in vitro and in vivo, using MTT and hematoxylin assays, respectively. Vitexin, quercetin O-galloyl-hexoside, and kaempferol O-acetyl-hexosyl-rhamnoside were referred to in this species for the first time. AeI and mainly AePF (Ae polyphenolic fraction) showed a significant antiradical activity against all tested radicals. Both AeI and AePF decreased NO levels in vitro, AePF being more active than AeI. In vivo anti-inflammatory and analgesic activities were verified for both samples at concentrations devoid of toxicity. Agrimony infusion and, mainly, AePF are potential sources of antiradical and anti-inflammatory polyphenols.
info:eu-repo/semantics/publishedVersion

openAccess
http://creativecommons.org/licenses/by/4.0/

eng