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A new C15-acetogenin, sagonenyne (20), exhibiting an unusual single tetrahydropyran ring was isolated from an ethyl acetate extract of Laurencia obtusa collected on the Corsican coastline. Its structure was established by detailed NMR spectroscopic analysis, mass spectrometry, and comparison with literature data. Twenty-three known compounds were identified in the same extract by means of column chromatography steps, using a (13)C-NMR computer aided method developed in our laboratory. In addition to sesquiterpenes, which represent the main chemical class of this extract, diterpenes, sterols, and C15-acetogenins were identified. The crude extract was submitted to a cytotoxicity assay and was particularly active against THP-1 cells, a human leukemia monocytic cell line.

The main objective of this study was the validation of the Healthy Lifestyle Questionnaire (EVS II), using a confirmatory factorial analysis of the measurement model, with veteran athletes. A total of 348 veteran Portuguese athletes of both genders, aged between 30 and 60 years (M = 41.64, SD = 9.83), of whom 200 were males and 148 were females, from several sports. The results of the confirmatory factor analysis demonstrate the adequacy of the adapted version of the EVS II, as the factorial structure (6 factors/24 items) has acceptable validity indexes: χ2 = 305.925, p = 0.000, df = 120.017, χ2/df = 2.549, NFI (Normed Fit Index) = 0.909, TLI (Tucker Lewis Index) = 0.918, CFI (Comparative Fit Index) = 0.944, GFI (Goodness of Fit Index) = 0.944, AGFI (Adjusted Goodness of Fit Index) = 0.909, SRMR (Standardized Root Mean Square Residual) = 0.048, RMSEA (Root Mean Square of Approximation) = 0.060, allowing evaluation of the dimensions of balanced diet, respect for mealtime, tobacco and alcohol consumption, other drugs consumption and resting habits. The adaptation to sport of the Portuguese version of EVS II can be used with reasonable confidence in the evaluation of healthy lifestyles in the context of sport

The Opuntia spp., most likely few individuals, were introduced in the Iberian Peninsula in the beginning of the 16th century, after the discovery of America, spreading afterwards throughout the Mediterranean basin. We analysed, for the first time, the genetic diversity in a set of 19 Portuguese Opuntia spp. populations from the species O. ficus-indica, O. elata, O. dillenii and O. robusta using nuclear microsatellite (nuSSR) markers. The Italian cultivars ‘Bianca’, ‘Gialla’ and ‘Rossa’ were included in the study for comparison purposes. The nuSSR amplifications produced from five to 16 alleles, with an average of 9.2 alleles per primer pair, and average polymorphism information content of 0.71. The estimated Dice coefficient among populations varied from 0.26 to 1.0, indicating high interspecific genetic diversity but low genetic diversity at the intraspecific level. The hierarchical clustering analysis revealed four major groups that clearly separated the four Opuntia species. Among the O. ficus-indica populations, two sub-clusters were found, one including the white pulp fruits (with cv. Bianca) and the other with the orange pulp ones and including the cv. Gialla, the cv. Rossa, and one pale yellow pulp population. No genetic differences were found between the inermis form, O. ficus-indica f. ficus-indica, and the rewilded spiny one, O. ficus-indica f. amyclaea. The dendrogram indicated that the clustering pattern was unrelated to geographical origin. The results revealed a low level of genetic diversity among the Portuguese populations of O. ficus-indica.