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“This is a post-peer-review, pre-copyedit version of an article published in Environmental Geochemistry and Health. The final authenticated version is available online at: https://doi.org/10.1007/s10653-018-0124-x "

In Opuntia spp., the cladode tissues contain many polysaccharides and secondary metabolites that interfere with obtaining high-quality deoxyribonucleic acid (DNA), using currently available methods. To circumvent this problem, three commercial kits, three modified versions of the conventional cetyltrimethylammonium bromide method (CTAB) method and one combined method were tested in Opuntia ficus-indica, O. robusta, O. dillenii and O. elata species. We obtained a rapid and simple protocol that allows the extraction of DNA from all the tested species with good DNA yield and purity, namely, the combined method. With this method (DNeasy® Plant Mini Kit combined with the CTAB method), DNA yields from 13.2 ± 7.8 to 15.9 ± 11.3 µg.g-1 of fresh tissue were obtained in the four Opuntia species. The purity, evaluated by the ratio A260/A280 ratio, ranged from 1.67 ± 0.12 to 2.01 ± 0.25, revealing low levels of problematic metabolites. The extracted DNA quality was confirmed by amplifying a set of nuclear microsatellites obtained for the genus. Reliable reproducible bands and electropherogram profiles were obtained. The combined method has potential to be universal for good-quality DNA extraction in cacti, particularly in the Opuntia genus and other difficult-to-extract species.

The present study aimed for the adaptation and validation of the Questionnaire Atest-EF, with the use of a confirmatory factorial analysis (CFA) of the measurement model, applied to students of the 3rd cycle of Basic Education. A total of 156 students of both genders, aged between 12 and 16 years (M = 14.7 ± SD = 1.3) were included in the study, with 84 female and 71 males, from the 7th, 8th and 9th grade. The main results reveal that the psychometric qualities confirm the adequacy of this model, demonstrating that the Atest-EF factorial structure: 1 factor/4 items, has very acceptable indexes of Atest - EF validity: (χ2 = 6.141, p = .000, χ2/gl = 3.07, NFI = 0.90 CFI = .925, IFI = .930, MFI = .9997, GFI = .995, AGFI = .975, RMR = .042, RMSEA = .08, SRMR = .028). With these results, the structural model reveals a satisfactory factorial assessment. This led to conclude that the Portuguese version of Atest-EF can be used, with high confidence in the evaluation of the student’s attention profiles in Physical Education classes. Compared with the moments of the class, there were only significant differences in students’ attention in the approach to sports in the first and fourth moments of the class, as well as in the general profile of attention, with significantly higher values in the approach to team sports.

“This is a post-peer-review, pre-copyedit version of an article published in Journal of Intelligent & Robotic Systems. The final authenticated version is available online at: https://doi.org/10.1007/s10846-019-01107-w”.