DABKOWSKA, Karolina (2017) - Bioactive compounds in olive oil : evaluation of hydroxytyrosol and tyrosol. Castelo Branco : IPCB. ESA. 1 CD-ROM. Relatório do Trabalho de Fim de Curso de Engenharia Biológica e Alimentar.
Title
Bioactive compounds in olive oil : evaluation of hydroxytyrosol and tyrosol
Contributor
Peres, Maria de Fátima Pratas
Subject
Phenolic alcohols Virgin olive oil HPLC-DAD Spectroscopy Total phenols
Date
2018-05-01T22:53:53Z 2018-05-01T22:53:53Z 2017
Format
application/pdf
Description
Disponível na Biblioteca da ESACB na cota C30-28954TFCEBA. In order to preserve the positive image of EVOO due to its health properties, as well as to quantify biophenols according to the health claim (EU Reg 432/2012) it is necessary to have an analytical protocol to evaluate the amount of hydroxytyrosol and its derivatives having a demonstrated effect of protection of blood lipids from oxidative stress as well as to check by this protocol if EVOOs satisfy the EU requirement for including the specific health claim on the oil label. Hydroxytyrosol and tyrosol are present in olive oil free and bound. The present work apply acid hydrolysis of olive oil total phenols with posterior analysis by HPLC-DAD. Total phenols is determined by acid hydrolysis-HPLC and by the VIS spectroscopy Folin Ciocalteu method (after and without the hydrolysis of the polar phenolic extract). The results showed that the FC assay do not significantly differ from those obtained following the acid hydrolysis-HPLC (expressed as sum of HTyr and Tyr).
