Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard
Type
article
Creator
Publisher
Identifier
FERNANDES, L. [et al.] (2008) - Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard. Ann. For. Sci. ISSN 1286-4560. 65.
1286-4560
Title
Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard
Subject
Genetic variation
Mating system
Gene flow
Simple sequence repeats
Maritime pine
Mating system
Gene flow
Simple sequence repeats
Maritime pine
Date
2011-05-24T16:00:30Z
2011-05-24T16:00:30Z
2008
2011-05-24T16:00:30Z
2008
Description
The original
publication is available at/A publicação original encntra-se disponível em: www.afs-journal.org
Relatedness among parents, variation in clonal fertility and background pollination deviate the realized genetic gain and the gene diversity of open pollinated seed orchard from expectation, in particular in wind pollinated species such as Pinus pinaster Aiton. • This work investigates the genetic variation, the mating system and the pollen contamination in a P. pinaster clonal seed orchard (CSO), by screening the 60 clones from the CSO and the seeds collected from 21 mother-trees with three nuclear microsatellites. • The expected diversity was similar, but the observed heterozygosity decreased 20% in the progenies compared with the parental trees. The outcrossing rate was 90.1%, the biparental inbreeding 21.7% computed through a multilocus approach, and the observed selfing 3.9%. The observed gene flow from outside the CSO was 52.4%. • From the results we concluded that the observed gene flow and the biparental inbreeding were high, and care should be taken in the implementation and management of future CSO, in particular clones should be checked for relatedness and the ramet number could be directly proportional to their breeding value.
L Fernandes was supported by a PRODEP III – Action 3.2 fellowship and the research was supported by ITQB research line ICL38. Special thanks are due to A. Aguiar for exchanging stimulating ideas and for his help in interpreting the data. This work could not have been done without the collaboration of M. Veloso from Estação Agronómica Nacional, who facilitated access to the sequencing unit. M.O. Krause is gratefully acknowledged for the final English revision.
Relatedness among parents, variation in clonal fertility and background pollination deviate the realized genetic gain and the gene diversity of open pollinated seed orchard from expectation, in particular in wind pollinated species such as Pinus pinaster Aiton. • This work investigates the genetic variation, the mating system and the pollen contamination in a P. pinaster clonal seed orchard (CSO), by screening the 60 clones from the CSO and the seeds collected from 21 mother-trees with three nuclear microsatellites. • The expected diversity was similar, but the observed heterozygosity decreased 20% in the progenies compared with the parental trees. The outcrossing rate was 90.1%, the biparental inbreeding 21.7% computed through a multilocus approach, and the observed selfing 3.9%. The observed gene flow from outside the CSO was 52.4%. • From the results we concluded that the observed gene flow and the biparental inbreeding were high, and care should be taken in the implementation and management of future CSO, in particular clones should be checked for relatedness and the ramet number could be directly proportional to their breeding value.
L Fernandes was supported by a PRODEP III – Action 3.2 fellowship and the research was supported by ITQB research line ICL38. Special thanks are due to A. Aguiar for exchanging stimulating ideas and for his help in interpreting the data. This work could not have been done without the collaboration of M. Veloso from Estação Agronómica Nacional, who facilitated access to the sequencing unit. M.O. Krause is gratefully acknowledged for the final English revision.
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Language
eng
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