Detection of Mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (Cervus elaphus) in Portugal: Evaluation of different methods
Type
article
Creator
Publisher
Identifier
MATOS, A.C., Luis FIGUEIRA, Maria H. MARTINS, Manuela MATOS, Sofia ÁLVARES, Andreia MENDES, Maria L. PINTO, Ana C. COELHO, Detection of Mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (Cervus elaphus) in Portugal: Evaluation of different methods, Journal of Veterinary Medical Science, 2017, Volume 79, Issue 3, Pages 692-698, Released March 28, 2017, [Advance publication] Released January 30, 2017, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.16-0153, https://www.jstage.jst.go.jp/article/jvms/79/3/79_16-0153/_article/-char/en,
Title
Detection of Mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (Cervus elaphus) in Portugal: Evaluation of different methods
Subject
Histopathology
Kidney
Mycobacterium avium subsp. paratuberculosis
PCR
Red deer
Kidney
Mycobacterium avium subsp. paratuberculosis
PCR
Red deer
Date
2019-11-22T23:57:42Z
2019-11-22T23:57:42Z
2017
2019-11-22T23:57:42Z
2017
Description
Paratuberculosis or Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. The present work is part of a wider set of studies designed to assess the prevalence of paratuberculosis in free ranging red deer (Cervus elaphus). With that purpose, 877 free-ranging red deer legally hunted in the Centre-eastern Portugal were submitted to necropsy and sampled for molecular methods, microbiology and histopathology. Thirty-seven (4.2%) kidneys revealed acid-fast bacilli when screened with the Ziehl-Neelsen technique. Map was detected by IS900 polymerase chain reaction (PCR) in thirty (81.1%) of the Ziehl-Neelsen positive kidneys. Subsequent PCR and/or culture from the different organs of the 37 examined animals allowed us to detect 86.4% (32 animals) infected red deer. Our results suggest that renal involvement in Map infected deer may be underdiagnosed and thus the routine examination of this organ and its inclusion in PCR techniques designed for Map detection could substantially improve the diagnostic of paratuberculosis in red deer.
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/publishedVersion
Access restrictions
restrictedAccess
Language
eng
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