Anti-Forssman prevalence in a sample of the portuguese population and its characterization
Type
article
Creator
Publisher
Identifier
MOURATO, Cristiana [et al.] (2021) - Anti-Forssman prevalence in a sample of the portuguese population and its characterization. HIGEIA : Revista Científica da Escola Superior de Saúde Dr. Lopes Dias. ISSN 2184-5565. Ano III, vol. 5, n.º 1, p. 59-68.
2184-5565
Title
Anti-Forssman prevalence in a sample of the portuguese population and its characterization
Prevalência de anti-Forssman numa amostra de população portuguesa e a sua caracterização
Prevalência de anti-Forssman numa amostra de população portuguesa e a sua caracterização
Subject
Forssman antigen
Antibodies
Blood group
Immunoglobulin
Transfusion safety
Antibodies
Blood group
Immunoglobulin
Transfusion safety
Date
2021-06-11T15:27:47Z
2021-06-11T15:27:47Z
2021
2021-06-11T15:27:47Z
2021
Description
Introdução
Descoberto em 1911 por Frederick Forssman, a
expressão do antigénio (Ag) Forssman (Fs) varia
entre espécies, estando raramente presente nos
eritrócitos humanos. Em 1987, três famílias inglesas
sem qualquer relação entre si, foram identificadas
com um fenótipo designado Apae que, mais tarde,
foi classificado como o 31º grupo sanguíneo: FORS.
Os anticorpos (Ac) anti-Fs têm ocorrência natural em
humanos e podem ter implicações transfusionais e
de transplante, uma vez que o Ag está presente na
superfície de eritrócitos, fluídos corporais e órgãos. Objetivos
O principal objetivo deste trabalho foi avaliar a
prevalência de Ab anti-Fs e esclarecer o seu impacto
na medicina transfusional, classificando o tipo de
imunoglobulina (Ig) envolvida.
Materiais e Métodos
Neste estudo foi utilizada a técnica standard de tubo
para avaliar a presença de Ac anti-Fs em amostras de
plasma de uma população de dadores Portugueses
e classificar as imunoglobulinas envolvidas. Foi
utilizada, para a realização de todas as experiências,
uma suspensão de eritrócitos de ovelha a 3-5% com
expressão positiva para Ag Fs.
Resultados
De um total de 11877 amostras, 117 (0,99%)
apresentaram reações fracas (entre 0 e 1 numa
escala de 0 a 4) quando em contacto com o Ag Fs
presente na suspensão de eritrócitos de ovelha.
Essas amostras foram posteriormente estudadas
quanto à presença da mutação no gene GBGT1,
responsável pela expressão da enzima Fs sintetase.
Das 192 amostras estudadas (50 de cada grupo
sanguíneo, à exceção do AB – o mais raro), para
classificar o Ac envolvido, 52% revelaram ser apenas
IgM, sendo as demais, uma mistura entre IgG e IgM. Conclusão
Na população estudada, poucas amostras (<1%)
apresentaram reação negativa contra os eritrócitos
de ovelhas, confirmando a baixa prevalência
deste grupo sanguíneo. Os padrões de reação das
amostras são independentes do grupo sanguíneo
ABO e do género. O Ac anti-Fs é principalmente, mas
não exclusivamente, IgM.
Introduction Discovered in 1911 by Frederick Forssman, the Forssman (Fs) antigen (Ag) expression varies among species, being rarely present on human red blood cells (RBC). In 1987, three unrelated English families were identified with a phenotype designed Apae which was later classified as the 31st blood group: FORS. The antibodies (Ab) anti-Fs have natural occurrence in humans and can interfere in transfusion and transplantation once the Ag is present on the surface of RBC, body fluids and organs. Goals The main goal of this research was to evaluate the prevalence of anti-Fs Ab and clarify its impact on transfusion medicine by classifying the type of immunoglobulin (Ig) involved. Materials and Methods In this study, standard tube technique was used to evaluate the presence of Ab anti-Fs in plasma samples from a Portuguese donor population and classify the immunoglobulin involved. It was used a 3-5% RBC sheep suspension with a positive expression for Fs Ag to perform all the experiments. Results From a total of 11877 samples, 117 (0,99%) showed weak reactions (between 0 and 1 on a scale from 0 to 4) when in contact with the Fs Ag present in the sheep RBC suspension. These samples would be further studied for the search of the mutation in the GBGT1 gene responsible for the expression of Fs synthase. From the 192 samples studied (50 from each blood type, except AB – the rarest) to classify the Ab involved, 52% revealed to be only IgM, being the remaining samples a mixture between IgG and IgM. Conclusion In the population studied, only a few samples (<1%) revealed a negative reaction against the sheep RBC, thus confirming the low prevalence of this blood group. The reaction patterns in the samples are independent of the ABO blood group and gender. The Ab against Fs is mainly, but not exclusively, IgM
info:eu-repo/semantics/publishedVersion
Introduction Discovered in 1911 by Frederick Forssman, the Forssman (Fs) antigen (Ag) expression varies among species, being rarely present on human red blood cells (RBC). In 1987, three unrelated English families were identified with a phenotype designed Apae which was later classified as the 31st blood group: FORS. The antibodies (Ab) anti-Fs have natural occurrence in humans and can interfere in transfusion and transplantation once the Ag is present on the surface of RBC, body fluids and organs. Goals The main goal of this research was to evaluate the prevalence of anti-Fs Ab and clarify its impact on transfusion medicine by classifying the type of immunoglobulin (Ig) involved. Materials and Methods In this study, standard tube technique was used to evaluate the presence of Ab anti-Fs in plasma samples from a Portuguese donor population and classify the immunoglobulin involved. It was used a 3-5% RBC sheep suspension with a positive expression for Fs Ag to perform all the experiments. Results From a total of 11877 samples, 117 (0,99%) showed weak reactions (between 0 and 1 on a scale from 0 to 4) when in contact with the Fs Ag present in the sheep RBC suspension. These samples would be further studied for the search of the mutation in the GBGT1 gene responsible for the expression of Fs synthase. From the 192 samples studied (50 from each blood type, except AB – the rarest) to classify the Ab involved, 52% revealed to be only IgM, being the remaining samples a mixture between IgG and IgM. Conclusion In the population studied, only a few samples (<1%) revealed a negative reaction against the sheep RBC, thus confirming the low prevalence of this blood group. The reaction patterns in the samples are independent of the ABO blood group and gender. The Ab against Fs is mainly, but not exclusively, IgM
info:eu-repo/semantics/publishedVersion
Access restrictions
openAccess
http://creativecommons.org/licenses/by/4.0/
http://creativecommons.org/licenses/by/4.0/
Language
eng
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